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KMID : 1177720140080010022
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Journal of Alternatives to Animal Experiments
2014 Volume.8 No. 1 p.22 ~ p.29
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Involvement of Intracellular Ca2+ Dynamics in Surfactant-induce Itch
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Choi Ji-Yei
Cho Sun-A
Kim Hyun-Na
Kim Ji-Hoon
Jang Won-Hee
Lee John-Hwan
An Su-Sun
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Abstract
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Itching is the unpleasant sense that urges to scratch the skin, and appears to vary according to the individual for the same stimulus. In recent animal studies, it has been reported that itching is caused by the use of anionic surfactants. Therefore, the itching sensation by ingredients and wash-off products containing various surfactants was assessed by use of in vitro method in this study. The change of intracellular Ca2+ level which included in the itch-inducing pathway was measured in human keratinocyte cell line (HaCaT) and human neuroblastoma cell line (SH-SY5Y). The influx of Ca2+ was increased in anionic surfactants and amphoteric surfactants rather than nonionic surfactants. In addition, according to increasing of anionic surfactants in wash-off products, intracellular Ca2+ level was elevated. This study could be useful for the development of safe wash-off products.
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KEYWORD
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surfactant, itch, intracellular calcium, human keratinocyte, human neuroblastoma cell
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